Staff Scientist - Development of CRISPR gene editing techniques

5 days left

Wellcome Trust Sanger Institute
GBP31498 - GBP39729 per annum + excellent benefits
28 Jul 2017
25 Aug 2017
Contract Type
Full Time

Salary range from GBP31,498 to GBP39,729 per annum plus excellent benefits.

We are seeking an enthusiastic, motivated staff scientist to develop and improve methods for genetic engineering in human induced pluripotent stem cells (iPSCs) using CRISPR/Cas9 technology. They will work as part of the human gene editing team within the cellular operations division, in the research and development section led by Dr Andrew Bassett.

The development of human iPSCs coupled with the use of genome editing technologies such as CRISPR/Cas9 has enabled us to introduce or correct the putative genetic lesions underlying many human diseases and analyse their cellular phenotype in an appropriate differentiated cell type. The WTSI has an exceptional background in human disease genetics and has recently produced hundreds of iPSCs derived from human patients, as well as more than 150 gene knockout iPSC lines.

The applicant will have the opportunity to apply and develop cutting edge genome engineering techniques, including the introduction of scarless point mutations, methods to bias DNA repair outcomes and the use and analysis of CRISPR libraries for forward genetic screens. This will allow screening and validation of the causative lesions underlying genetic diseases. They will also develop technologies for phenotypic characterisation of the resulting cell lines to understand the role of such genetic variation in cellular phenotypes. Together, this will allow the identification of causative genetic aberrations underlying disease states as well as a mechanistic understanding of their function at a cellular level.

Candidates with a PhD in a relevant biological discipline with a strong background in genome engineering and/or human iPSC culture are strongly encouraged to apply. Excellent cell culture and molecular biology experience, a strong track record of publication and an ability to work both independently and as part of a team are essential. The post-holder will work closely with both the human gene editing groups at WTSI and with a number of other members of the scientific faculty at the institute.

Essential Skills

  • PhD degree and/or postdoctoral training in genetics, molecular or cellular biology or a similar subject
  • Significant proven experience of mammalian cell culture
  • Practical experience with genome engineering (e.g. CRISPR, TALEN) ideally in human ES or iPS cells
  • Significant experience in molecular biology and advanced cloning techniques (e.g. recombineering, golden gate or Gibson assembly)
  • Experience with generation and/or computational analysis of genomics datasets (e.g. RNAseq, ATACseq, ChIPseq, sgRNA screening)
  • Strong track record of publications
  • In depth knowledge of the field of stem cell biology and genome engineering
  • A thorough understanding of genome browsers and gene targeting technologies
  • Ability to work independently on projects to the point of publication, as well as part of a team
  • Excellent written and verbal communication skills
  • Ability to test and implement new assays and techniques including their troubleshooting

Ideal Skills

  • Stem cell culture techniques especially in human ES or iPS cells
  • Experience of generation of sgRNA libraries or other high-throughput cloning
  • Scripting experience (e.g. Perl, Python, R, etc.) for computational analysis of large datasets

Other information

Please include a covering letter and CV with your application.

Closing date for applications: 17th August 2017, however applications will be considered and reviewed on an on-going basis and therefore the post may be filled before the deadline.